TIE2/HEK293
CBP74161
詢 價
索取Protocol
產(chǎn)品描述
產(chǎn)品數(shù)據(jù)庫
| I. Background | |
| TIE2(酪氨酸激酶受體)主要表達(dá)于血管內(nèi)皮細(xì)胞表面的受體,胞外區(qū)含免疫球蛋白 域 (結(jié)合 ANG ) 、 EGF 樣域 (介導(dǎo)二聚化) ; 胞內(nèi)區(qū)含酪氨酸激酶活性位點(diǎn) (Y1100/Y1108)。被配體激活后通過 PI3K/AKT 和 MAPK 通路調(diào)控內(nèi)皮細(xì)胞存活、遷移 及血管屏障功能。在病理狀態(tài)下(如膿毒癥、腫瘤),TIE2 信號異常導(dǎo)致血管滲漏或異常 增生。 | |
| II. Description | |
| TIE2 HEK293 細(xì)胞,在 HEK293 細(xì)胞的基礎(chǔ)上,穩(wěn)定表達(dá)人 TIE2。 | |
| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | TIE2 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10% FBS+2 μg/ml Puromycin |
| Storage: | Liquid nitrogen |
| Application(s): | Binding Assay,FACS |
| IⅤ. Representative Data | |
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Figure 1. Recombinant TIE2/HEK293 constitutively expressing TIE2. |
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