CXCR2/CHO
CBP71357
询 价
索取protocol
产品描述
产品数据库
| I. Background | |
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趋化因子是一类炎性细胞因子，具有调节细胞迁移、粘附、吞噬作用、细胞因子分泌、 增殖和细胞凋亡的能力。越来越多的证据表明，趋化因子及其受体在稳态和炎症条件下在 调节树突状细胞迁移以及稳态和炎症功能方面发挥着重要作用。 |
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| II.Description | |
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cAMP 在 G 蛋白偶联受体（GPCR）信号转导中是一种关键的第二信使。当配体结合 到 GPCR，构象发生变化，激活受体接着激活 G 蛋白，Gi 的激活导致腺苷酸环化酶调控 cAMP 的下调。CXCR2 CHO 药靶模型很好的模拟了体内 CXCR2 的信号转导过程，原理见 下图所示。
Figure 1. CXCR2 CHO 细胞模型原理图
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| III. Introduction | |
| Host Cell: |
CHO |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5 μg/ml Puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for CXCR2 |
| Transducer: | Gi |
| IV. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| V. Representative Data | |
 Figure 2. Recombinant CXCR2/CHO constitutively expressing CXCR2. |
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Figure 3. HTRF cAMP Assay with CXCR2 CHO(C7). |
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